Water channel Aquaporin 4 is required for T cell activation.

Abstract Aquaporins are a family of ubiquitously expressed transmembrane water channels. We have previously reported that Aquaporin 4 (AQP4) is on T cells and treatment with small molecule AQP4 inhibitor significantly delays cell mediated heart allograft rejection. The goal this study was to identify mechanisms prolonged graft survival following blockade. Stimulation murine WT + AER-270 or −/−T resulted in decreased expression activation markers CD25 CD44, reduced volume when compared control cells. In addition, the absence inhibition also limited IL-2 production by stimulation. Intact required for optimal IFNg Analogously, ability human proliferate TCR then examined role signal transduction found nuclear translocation NF-KB NFAT calcium flux Either impaired as early phosphorylation Lck (pY394), ZAP70 (pY319) LAT (p171). shown associate actin cytoskeleton other types, we next tested its involvement polarization/capping. loss capping dysfunctional post-TCR stimulation remodeling kinetics. Our findings reveal novel underlying importance lymphocyte potential therapeutic target preventing TCR-mediated solid organ transplantation diseases.